研究紹介

1.Transbilayer distribution of lipids at nano scale
Transbilayer distribution of lipids at nano scale

Motohide Murate, and Toshihide Kobayashi
Lipid Biology Laboratory, RIKEN

さまざまな脂質結合プローブとフリーズフラクチャー免疫電子顕微鏡法を併用することにより形質膜内外層における脂質の非対称分布をナノメートルレベルで明らかにすることに成功した。

2.Development of non-target lipidomics platform
Development of non-target lipidomics platform

Kazutaka Ikeda, and Makoto Arita
Laboratory for Metabolomics, RIKEN Center for Integrative Medical Sciences

ノンターゲット解析を志向したリピドミクス新技術の開発

3.Changes in lipid metabolism during differentiation pathway of mammalian cells
Changes in lipid metabolism during differentiation pathway of mammalian cells

Sota Takanezawa, and Yasushi Sako
Cellular Informatics Laboratory, RIKEN

1細胞ラマンスペクトル計測により、細胞分化の初期過程における脂質その他の化学組成の活発な変動が観測された。

4.Protonation and charged states of amino-acid residues and ions in the
transmembrane region of an ion pump analyzed by electron crystallography
of thin 3D crystals
Protonation and charged states of amino-acid residues and ionsin the transmembrane region of an ion pumpanalyzed by electron crystallography of thin 3D crystals

Koji Yonekura
Biostructural Mechanism Laboratory, RIKEN SPring-8 Center

新規開発した電子線三次元結晶構造解析により、膜貫通領域のアミノ酸とイオンの荷電状態、側鎖の水素化状態が明らかにできた。

5.Lipidomics of glucosylated lipids in the CNS
Lipidomics of glucosylated lipids in the CNS

Kazuki Nakajima, and Yoshio Hirabayashi
Laboratory for Molecular Membrane Neuroscience, RIKEN Brain Science Institute

アミノ酸、L-セリンのスフィンゴ脂質代謝への影響を調べるために、新たな質量分析システムを構築した。その結果、細胞内セリンの減少が、細胞ストレスを誘導する生理活性質1-デオキシスフィンガニンの産生を促すことを発見した。

6.Neuron-Glia interaction mediated by Lyso-phosphatidylglucoside
Glia-neuron interaction mediated by lysoPtdGlc and its specific receptor, GPR55.

Yoshio Hirabayashi, Yeon-Jeong Kim, and Yasuko Nagastuka
Laboratory for Molecular Membrane Neuroscience, RIKEN Brain Science Institute

7.Regulatory mechanisms for the activation of group 2 innate lymphoid cells
Regulatory mechanisms for the activation of group 2 innate lymphoid cells

Kazuyo Moro1, Hiroki Kabata1,2, Satoshi Koga1, and Shigeo Koyasu3
1Laboratory for Innate Immune Systems, RIKEN IMS, 2Department of Medicine, Keio University School of Medicine, 3Laboratory for Immune Cell Systems, RIKEN IMS

8.Analysis of thermal-stress induced nucleocytoplasmic transport carrier Hikeshi

Naoko Imamoto
Cellular Dynamics Laboratory, RIKEN

9.Detailed distribution of sphingomyelin on the plasma membranes revealed by specific probes
Cell surface labeling of Lys and EqtII-GFP were estimated by calculating the density of gold particles on the plasma membrane sheets from normal and NPA fibroblasts.

Motohide Murate, and Toshihide Kobayashi
Lipid Biology laboratory, RIKEN

10. Membrane tubulation ability of phospholipase Cβ1 C terminal domain
Darkfield microscopy image of liposome tubulation induced by brain extract.

Takehiko Inaba, Takuma Kishimoto, Motohide Murate, Takuya Tajima, Shota Sakai, Mitsuhiro Abe, Asami Makino, Nario Tomishige,
Reiko Ishitsuka, Yasuo Ikeda, Shinji Takeoka, and Toshihide Kobayashi
Lipid Biology Laboratory, RIKEN